I don't add any eq during profiling or after. Some of the low end may be attributed to Fletcher Munson curves and volume. I find some of my profiles sound darker at lower volumes but sound balanced at high (stage) volume. Definitely, tweak the definition a bit with humbuckers. I find humbuckers have a wide range of tone and it's hard to "solve" for all of those variables on my end but that's why I try to find mic positions and settings where the Definition has room to change both up and down so it can be adjusted. Many high gain amps have a lot of high frequency distortion which makes the Definition really high but it can't be brightened that way after the fact using the Definition. My baseline humbucker has been a Gibson 57 Classic when profiling higher gain amps lately. It is more vintage voiced, so if you have hotter or warmer humbuckers you may have to tweak the Definition as well as use some EQ for a low cut to get it in balance. The 5150 pack seems to have a little clearer cut without the low end build up.
You can also try swapping cabs from other rigs if you find a cab that gets rid of the low end. It can sometimes work. I've done that a little bit on some hard to tweak rigs.
